| Gene | Scarf2 |
| Chromosome | 16 |
| Deletion Start | 17,797,365 |
| Deletion End | 17,807,715 |
| Deletion Size | 10,351 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 17,797,893 | 17,797,911 | AGAAGGGCGAAGGAGCAAC |
| TUP | 17,797,922 | 17,797,946 | CTGGGCACTGGGACAGTCGAGACTT |
| TUR | 17,797,954 | 17,797,976 | CCCGCTCTACCAAGACAAATCTG |
| TDF | 17,806,724 | 17,806,747 | TGTGATTAGCTTGTCATTCCCTTG |
| TDP | 17,806,748 | 17,806,770 | TTAGAAGCGACTGCCGACAGCCG |
| TDR | 17,806,791 | 17,806,810 | AGCGGCCACCTCAGTGAGAG |
PCR Primers
| Primer | Sequence |
| SU | AGTTCGTGCATGGCCAGGT |
| SD | CCATAGGCACCAGGCCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 371 bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | 317 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10731A-E8 | VGB6 | C57BL/6NTac | |
| 10731A-H6 | VGB6 | C57BL/6NTac | |
| 10731B-A1 | VGB6 | C57BL/6NTac | |
| 10731B-G4 | VGB6 | C57BL/6NTac | |
| 10731B-A6 | VGB6 | C57BL/6NTac | |
| 10731B-G8 | VGB6 | C57BL/6NTac | |
