| Gene | Prss1 |
| Chromosome | 6 |
| Deletion Start | 41,458,927 |
| Deletion End | 41,463,734 |
| Deletion Size | 4,808 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 41,458,954 | 41,458,972 | CCTGTTCCTGGCCCTTGTG |
| TUP | 41,458,975 | 41,459,000 | AGCTGCTGGTGAGTTCCATGCTTTCA |
| TUR | 41,459,032 | 41,459,052 | GCAGCCTTGGTGGTAAAGTTC |
| TDF | 41,463,294 | 41,463,314 | CCTAGAGGGAGGCAAGGATTC |
| TDP | 41,481,305 | 41,481,328 | CTGCCAGGTGATTGCCACTCTCCA |
| TDR | 41,463,354 | 41,463,374 | TTGAAGCTCAACTCCCAGAAC |
PCR Primers
| Primer | Sequence |
| SU | CCATCAGTCTAAGGTCAAGC |
| SD | CATCACTTTGCTCCTGTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 373 bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | 300 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10732A-G7 | VGB6 | C57BL/6NTac | |
| 10732A-A3 | VGB6 | C57BL/6NTac | |
