| Gene | Cst11 |
| Chromosome | 2 |
| Deletion Start | 148,771,400 |
| Deletion End | 148,768,671 |
| Deletion Size | 2,730 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 148,770,613 | 148,770,637 | GAGTCAGACAGGAAAGAAGAGCAAG |
| TUP | 148,770,579 | 148,770,606 | ACAACTGTGCAAGACTAGGCTCTTGACA |
| TUR | 148,770,550 | 148,770,569 | TGGGCAGGACAGCTTTACAC |
| TDF | 148,768,908 | 148,768,931 | GAGTCTAACTATGGAGCCCATTTC |
| TDP | 148,768,873 | 148,768,901 | TAAACCTCTCACCTCAGGTTCCCAATTGC |
| TDR | 148,768,837 | 148,768,857 | AACAAGAGCTGGGCATGGAAG |
PCR Primers
| Primer | Sequence |
| SU | GCTATGCAAAAAGATTGATAGTG |
| SD | GTCTCCTACTTGTTAGGAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 311 bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | 336 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10733A-D4 | VGB6 | C57BL/6NTac | |
| 10733A-H5 | VGB6 | C57BL/6NTac | |
| 10733A-F10 | VGB6 | C57BL/6NTac | |
