| Gene | Olfr71 |
| Chromosome | 4 |
| Deletion Start | 43,706,563 |
| Deletion End | 43,705,575 |
| Deletion Size | 989 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,706,475 | 43,706,495 | GCGGCTACTCTTTCCTCTGTG |
| TUP | 43,706,445 | 43,706,469 | TCATGTACCTGGTGACTCTGCTGGG |
| TUR | 43,706,427 | 43,706,444 | GCCACGATGGCTGTGTTC |
| TDF | 43,705,917 | 43,705,935 | TGACCCTCATCTGCCTGTC |
| TDP | 43,705,891 | 43,705,913 | CATGCTGATCCTGGCCACCATCC |
| TDR | 43,705,842 | 43,705,862 | GAGCAGGTGGAGAAGGCTTTG |
PCR Primers
| Primer | Sequence |
| SU | CAGACTTCCAAGTACAGTCG |
| SD | CTGGGTCTTACTATCACAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 334 bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | 403 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10734A-B12 | VGB6 | C57BL/6NTac | |
| 10734A-D7 | VGB6 | C57BL/6NTac | |
| 10734B-F2 | VGB6 | C57BL/6NTac | |
