| Gene | Tspan6 |
| Chromosome | X |
| Deletion Start | 133,898,291 |
| Deletion End | 133,892,674 |
| Deletion Size | 5,618 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 133,897,414 | 133,897,434 | GTGGCAACTGGGCTGTTAATC |
| TUP | 133,897,381 | 133,897,408 | TCACAAGCATTGTGTCTTAGATGCCTCC |
| TUR | 133,897,334 | 133,897,356 | GAAACAATCAGGCACCGTACAAG |
| TDF | 133,893,028 | 133,893,046 | TGGTAGCTGAGGGCTTCTC |
| TDP | 133,893,008 | 133,893,026 | AAATCCCAGGACTTTTGAA |
| TDR | 133,892,973 | 133,892,993 | GTGACCCGGAACTTCACAAAG |
PCR Primers
| Primer | Sequence |
| SU | TCAGTAACGATCAGTAGGGG |
| SD | GCTACATACATACTTTCAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 328 bp | - | + | + |
| TUF | TUR | 101 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | 302 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10736B-E7 | VGB6 | C57BL/6NTac | |
| 10736B-F8 | VGB6 | C57BL/6NTac | |
