| Gene | Inpp5e |
| Chromosome | 2 |
| Deletion Start | 26,408,584 |
| Deletion End | 26,397,797 |
| Deletion Size | 10,788 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 26,408,525 | 26,408,545 | GGGCAGCTAGAAGGCAGAATG |
| TUP | 26,408,502 | 26,408,523 | TCCAGGGACAGCCTCCGAACAC |
| TUR | 26,408,471 | 26,408,491 | GAAGCCTGGTGTTGGGATGAG |
| TDF | 26,398,238 | 26,398,259 | AGATGGGACGAAGAACCCTAAC |
| TDP | 26,398,214 | 26,398,236 | TCTGGGCAGTGGCCCATTTCCTA |
| TDR | 26,398,170 | 26,398,188 | GAGCTGGCCTCAACTTCAC |
PCR Primers
| Primer | Sequence |
| SU | CATGCAGCTACAGGCCCTC |
| SD | GCTCTAGCATGAGAACAGGA |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 328 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | 352 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10739D-A11 | VGB6 | C57BL/6NTac | |
| 10739D-C9 | VGB6 | C57BL/6NTac | |
| 10739D-H7 | VGB6 | C57BL/6NTac | |
