Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

10740 Pth2 Status: ES cell colonies screened / QC positives

Gene	Pth2
Chromosome	7
Deletion Start	45,181,278
Deletion End	45,181,781
Deletion Size	504
Genome Build	38

MGI ID	MGI:2152297
NCBI Gene ID	114640
BAC ID	406A8
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 7

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	45,181,328	45,181,347	AGCGGCTGTTGCTGCTTTTG
TUP	45,181,348	45,181,371	CTGCTGCTACTGCTTGTGCCCTGG
TUR	45,181,412	45,181,433	CCTCACACCTACCTGAACACAC
TDF	45,181,666	45,181,683	CTGACGACGCGGCCTTTC
TDP	45,181,697	45,181,715	CCTGCTGGCCGCCCTGGAG
TDR	45,181,735	45,181,756	AGCAACAGCTTCTGCATGTAAG

PCR Primers

Primer	Sequence
SU	GCTTCTCATTACCGCTGGC
SD	CCAAAACAGAGCTGGATCAG
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	361 bp	-	+	+
TUF	TUR	106 bp	+	+	-
TDF	TDR	91 bp	+	+	-
NeoFwd	SD	282 bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
10740A-A7	VGB6	C57BL/6NTac
10740A-A1	VGB6	C57BL/6NTac
10740A-A11	VGB6	C57BL/6NTac