| Gene | Olfr1371 |
| Chromosome | 11 |
| Deletion Start | 52,213,984 |
| Deletion End | 52,212,849 |
| Deletion Size | 1,136 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 52,213,828 | 52,213,852 | ATCATTCTGTCTCAACTGGACCTCT |
| TUP | 52,213,796 | 52,213,824 | TGCACACACCCATGTACTACTTTCTCTGC |
| TUR | 52,213,703 | 52,213,723 | TCCAAAGCTGATGGTCCTGTC |
| TDF | 52,213,226 | 52,213,247 | TGCCATGTTTTATGGCTCAGGT |
| TDP | 52,213,198 | 52,213,222 | TCCACATACCTCCAACCTGTCCACA |
| TDR | 52,213,177 | 52,213,197 | TCCCCTCCTTCTCAGAATACC |
PCR Primers
| Primer | Sequence |
| SU | CTTCCATTTCCTCTCTGAACC |
| SD | GGTCATACTTACTTAGAGAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 321 bp | - | + | + |
| TUF | TUR | 150 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | 318 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10741E-F11 | VGB6 | C57BL/6NTac | |
| 10741E-F10 | VGB6 | C57BL/6NTac | |
