| Gene | Caskin2 |
| Chromosome | 11 |
| Deletion Start | 115,811,977 |
| Deletion End | 115,799,969 |
| Deletion Size | 12,009 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 115,811,673 | 115,811,691 | CTGTGTCTCCGGGCAAGTC |
| TUP | 115,811,646 | 115,811,672 | ACTTAACAGCTCTGAACCACGGAGCCC |
| TUR | 115,811,604 | 115,811,627 | GCAGGAGCTGAATGTGTTTCAATG |
| TDF | 115,800,417 | 115,800,438 | GACTGGAACAGACCAGCTCATC |
| TDP | 115,800,392 | 115,800,411 | AAGCCGCACTGAGGGCAGCT |
| TDR | 115,800,372 | 115,800,391 | TCGGTGCCAATGCTCTTCTC |
PCR Primers
| Primer | Sequence |
| SU | GCTTTCCTGCCTGTGGTGG |
| SD | TTCGCTCCCACACAGCTGA |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 327 bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | 306 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10745B-C8 | VGB6 | C57BL/6NTac | |
| 10745C-B7 | VGB6 | C57BL/6NTac | |
| 10745C-A10 | VGB6 | C57BL/6NTac | |
