| Gene | C1ra |
| Chromosome | 6 |
| Deletion Start | 124,513,653 |
| Deletion End | 124,523,202 |
| Deletion Size | 9,550 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 124,514,340 | 124,514,361 | ATGCTCTTCCTCGCACTATGAG |
| TUP | 124,514,364 | 124,514,388 | TCAGGCAACAGCCAGGAGACTAAGA |
| TUR | 124,514,416 | 124,514,438 | GACTTGTTGAGTCCCCTTTGTTG |
| TDF | 124,522,229 | 124,522,249 | CACAGAAGGAGCGCATCATCA |
| TDP | 124,522,251 | 124,522,268 | AGGGCAGCCAGCCAGGCC |
| TDR | 124,522,324 | 124,522,343 | ATCCAGCGGTCTCCAAGCAG |
PCR Primers
| Primer | Sequence |
| SU | ACGAGAAACTCAGCTGCAG |
| SD | AAGATCCTTCCATGGCTACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 333 bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 115 bp | + | + | - |
| NeoFwd | SD | 348 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10746B-E2 | VGB6 | C57BL/6NTac | |
| 10746B-E3 | VGB6 | C57BL/6NTac | |
| 10746B-E8 | VGB6 | C57BL/6NTac | |
| 10746B-F7 | VGB6 | C57BL/6NTac | |
| 10746B-E4 | VGB6 | C57BL/6NTac | |
