| Gene | Tnnc2 |
| Chromosome | 2 |
| Deletion Start | 164,778,224 |
| Deletion End | 164,777,281 |
| Deletion Size | 944 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 164,777,825 | 164,777,846 | GGCAGCGGTACTATCGACTTTG |
| TUP | 164,777,797 | 164,777,821 | AGTTCTTGGTCATGATGGTGCGCCA |
| TUR | 164,777,776 | 164,777,796 | CCCTTCGCATCCTCTTTCATC |
| TDF | 164,777,623 | 164,777,643 | GACCCTGTGTCCTTCTCTGTC |
| TDP | 164,777,598 | 164,777,620 | TCCCTCCCAAAGGAACGCAGACG |
| TDR | 164,777,518 | 164,777,540 | CAGGGATTCGATCTCCTCTTCTG |
PCR Primers
| Primer | Sequence |
| SU | TCCCCGACTACTCTCTGAAG |
| SD | TAGCTCCAGCTGCTGGTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 325 bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 126 bp | + | + | - |
| NeoFwd | SD | 383 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10748B-B1 | VGB6 | C57BL/6NTac | |
| 10748B-C7 | VGB6 | C57BL/6NTac | |
| 10748B-D2 | VGB6 | C57BL/6NTac | |
| 10748B-D5 | VGB6 | C57BL/6NTac | |
| 10748B-E12 | VGB6 | C57BL/6NTac | |
| 10748B-G6 | VGB6 | C57BL/6NTac | |
