| Gene | Taar6 |
| Chromosome | 10 |
| Deletion Start | 23,985,643 |
| Deletion End | 23,984,609 |
| Deletion Size | 1,035 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 23,985,258 | 23,985,279 | TGAGCTTCATCTCCATCGACAG |
| TUP | 23,985,233 | 23,985,255 | ACATCGCTGTCACGGACCCTCTG |
| TUR | 23,985,211 | 23,985,232 | CCGTGAACTTGGTGGGATAGAC |
| TDF | 23,984,738 | 23,984,761 | TGTGTTTGGTGTGCTTATTACAAC |
| TDP | 23,984,712 | 23,984,737 | TCAGCCATGAACCCTCTGATCTACGC |
| TDR | 23,984,630 | 23,984,651 | GTTCATAGTGGCTGAGCTGTTC |
PCR Primers
| Primer | Sequence |
| SU | CCATTATTTTGGACCATAGGAGC |
| SD | GTTCAGCTCTGAGAAGAGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 132 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10749A-D5 | VGB6 | C57BL/6NTac | |
| 10749A-D6 | VGB6 | C57BL/6NTac | |
| 10749A-E2 | VGB6 | C57BL/6NTac | |
| 10749A-G3 | VGB6 | C57BL/6NTac | |
| 10749A-H10 | VGB6 | C57BL/6NTac | |
| 10749A-H11 | VGB6 | C57BL/6NTac | |
