| Gene | Fgb |
| Chromosome | 3 |
| Deletion Start | 83,049,758 |
| Deletion End | 83,042,025 |
| Deletion Size | 7,734 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 83,049,015 | 83,049,034 | GTGTAGGTCTGGCTGTCCTG |
| TUP | 83,048,982 | 83,049,008 | AGACCAGACTCACAGTGATCTGCCTGC |
| TUR | 83,048,917 | 83,048,938 | GCAACGACAAGGCTGAGACAAG |
| TDF | 83,042,572 | 83,042,592 | TGGTGGCTGGTGGTATAACAG |
| TDP | 83,042,547 | 83,042,570 | TGCCATGCAGCCAATCCAAATGGC |
| TDR | 83,042,477 | 83,042,497 | TCCACACCACACCATCATCTG |
PCR Primers
| Primer | Sequence |
| SU | CAGTGAGGAAGAGCTGTCTG |
| SD | TTACAATGGCAGAGATGGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 366 bp | - | + | + |
| TUF | TUR | 118 bp | + | + | - |
| TDF | TDR | 116 bp | + | + | - |
| NeoFwd | SD | 334 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10750A-H9 | VGB6 | C57BL/6NTac | |
| 10750D-B1 | VGB6 | C57BL/6NTac | |
