| Gene | Pcdhb22 |
| Chromosome | 18 |
| Deletion Start | 37,518,484 |
| Deletion End | 37,520,926 |
| Deletion Size | 2,443 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 37,519,193 | 37,519,215 | CTGAGTTTGCACAGGCTTTCTAC |
| TUP | 37,519,220 | 37,519,246 | TGCAAATTCCAGAGAACAGTCCCTCGG |
| TUR | 37,519,282 | 37,519,302 | CTCTCCATTTGTCCCAGTGTC |
| TDF | 37,519,962 | 37,519,980 | ACAGCTGGCCCTCAACTCA |
| TDP | 37,309,143 | 37,309,168 | TCATCTCCATCAACGCTGACAACGGG |
| TDR | 37,520,065 | 37,520,083 | CTCGGTCTGTGGCACTCAC |
PCR Primers
| Primer | Sequence |
| SU | AGAGCATAGTCAACGCATGG |
| SD | GGATCCAACCTAAGGCCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 358 bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 122 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10751D-H4 | VGB6 | C57BL/6NTac | |
| 10751E-B5 | VGB6 | C57BL/6NTac | |
| 10751E-B7 | VGB6 | C57BL/6NTac | |
| 10751E-A4 | VGB6 | C57BL/6NTac | |
| 10751A-D4 | VGB6 | C57BL/6NTac | |
| 10751B-C5 | VGB6 | C57BL/6NTac | |
| 10751C-E9 | VGB6 | C57BL/6NTac | |
