| Gene | Akr1b7 |
| Chromosome | 6 |
| Deletion Start | 34,412,394 |
| Deletion End | 34,422,889 |
| Deletion Size | 10,496 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,412,939 | 34,412,961 | GAGAGCCACAGAATTGGAGTCAG |
| TUP | 34,412,963 | 34,412,989 | TCAAAGTCTGCCCTGGCTGTGTTAATC |
| TUR | 34,413,015 | 34,413,037 | CTGTTTCACTGGGTACTGGGATG |
| TDF | 34,422,612 | 34,422,632 | GAACTGGTGACCGAGTGACTG |
| TDP | 34,422,635 | 34,422,657 | AGCTCGCAGGCCTTCCTTCCTTA |
| TDR | 34,422,678 | 34,422,698 | GGCACACAACAGGACCTTCTC |
PCR Primers
| Primer | Sequence |
| SU | GCCTTTGCACTGTGCTGAC |
| SD | AATGGACAGCATACTACAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 335 bp | - | + | + |
| TUF | TUR | 99 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | 355 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10752A-G8 | VGB6 | C57BL/6NTac | |
| 10752A-C10 | VGB6 | C57BL/6NTac | |
| 10752A-D7 | VGB6 | C57BL/6NTac | |
