| Gene | Tmem97 |
| Chromosome | 11 |
| Deletion Start | 78,550,650 |
| Deletion End | 78,542,482 |
| Deletion Size | 8,169 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 78,550,118 | 78,550,138 | TGGTTAGTGCTCGTGGCATTC |
| TUP | 78,550,090 | 78,550,115 | ACACTCCTTCTGGGTCTGGATCTAGC |
| TUR | 78,550,065 | 78,550,087 | CGACATTTCCTCAACTGCTCCTC |
| TDF | 78,542,948 | 78,542,969 | TGAGCTGTTTGGGTCCTGTAAG |
| TDP | 78,542,916 | 78,542,938 | TGAGCAGCGCAACACCTGGTTTC |
| TDR | 78,542,864 | 78,542,882 | GCCTCATCACCCGTTTGTC |
PCR Primers
| Primer | Sequence |
| SU | GCAGCCAATGGAAACAACAG |
| SD | TCCAGGTCAGGAGCTAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 349 bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | 302 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10753A-D5 | VGB6 | C57BL/6NTac | |
| 10753A-H8 | VGB6 | C57BL/6NTac | |
| 10753B-C11 | VGB6 | C57BL/6NTac | |
| 10753B-C12 | VGB6 | C57BL/6NTac | |
| 10753B-G3 | VGB6 | C57BL/6NTac | |
| 10753B-D2 | VGB6 | C57BL/6NTac | |
| 10753B-F12 | VGB6 | C57BL/6NTac | |
