| Gene | Clcf1 |
| Chromosome | 19 |
| Deletion Start | 4,214,471 |
| Deletion End | 4,222,604 |
| Deletion Size | 8,134 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,214,553 | 4,214,571 | AGATCCGGTTCCCGTCCTC |
| TUP | 4,214,576 | 4,214,595 | CCACCCTCGGAGCCCCAAGC |
| TUR | 4,214,701 | 4,214,719 | CGTTCTCAGCCACCTCCTG |
| TDF | 4,222,171 | 4,222,189 | GGCGAAGCCTCAATGACAG |
| TDP | 4,222,194 | 4,222,217 | CGGCTGACCCAGAACTATGAGGCG |
| TDR | 4,222,230 | 4,222,248 | GGCCACGCAAGTAACACAG |
PCR Primers
| Primer | Sequence |
| SU | GAGGAGCCCCAGAAGCCCT |
| SD | GCCTCGATGCCCACTGTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 392 bp | - | + | + |
| TUF | TUR | 167 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | 256 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10755A-H8 | VGB6 | C57BL/6NTac | |
| 10755A-A10 | VGB6 | C57BL/6NTac | |
| 10755A-D4 | VGB6 | C57BL/6NTac | |
| 10755A-F7 | VGB6 | C57BL/6NTac | |
| 10755A-G9 | VGB6 | C57BL/6NTac | |
