| Gene | Lpar4 |
| Chromosome | X |
| Deletion Start | 106,930,212 |
| Deletion End | 106,931,324 |
| Deletion Size | 1,113 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 106,930,559 | 106,930,577 | TGCGTTCCTCACCAACATC |
| TUP | 106,930,586 | 106,930,613 | CATGCTCTTCCTCACCTGCATCAGTGTG |
| TUR | 106,930,618 | 106,930,642 | AAGGGATAGACAATGGCTAGGAAAC |
| TDF | 106,930,836 | 106,930,860 | GAAGTTGTTGGATTCATCATTCCTC |
| TDP | 106,930,874 | 106,930,902 | TTCTTGTTCTTCTGTGGTGCTTAGAACCC |
| TDR | 106,930,907 | 106,930,927 | TGAGACAATGTTGCAGGCTTG |
PCR Primers
| Primer | Sequence |
| SU | TGCGTGATCTCTGCCATATCC |
| SD | ACCCTCACATCACTAAAAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 406 bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | 362 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10756D-A8 | VGB6 | C57BL/6NTac | |
| 10756D-F7 | VGB6 | C57BL/6NTac | |
