10757   Arhgef15     Status: ES cell colonies screened / QC positives

GeneArhgef15
Chromosome11
Deletion Start68,955,021
Deletion End68,944,565
Deletion Size10,457
Genome Build38
MGI IDMGI:3045246
NCBI Gene ID442801
BAC ID133M18
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF68,954,16568,954,186GCTTAGGCAATCCCTTCTTCAC
TUP68,954,14368,954,164AGATGCTGCCCTGGCCTGCCCT
TUR68,954,11268,954,131TTGCTACATGGCAGACACAG
TDF68,945,42468,945,444TGCTGTGCAATAGGAACTGTC
TDP68,945,39968,945,420AGGCCAGTGTGCCCAGACTGAC
TDR68,945,37668,945,397GCTTCCATCATTCCCTTCTCTG

PCR Primers

PrimerSequence
SUGGAAAAGCTGCCCAGGAAG
SDATGCCCCTGCTTAATCTTCC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev402 bp-++
TUFTUR75 bp++-
TDFTDR69 bp++-
NeoFwdSD316 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
10757A-D2VGB6C57BL/6NTac
10757B-C6VGB6C57BL/6NTac
10757B-G3VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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