| Gene | Wfdc1 |
| Chromosome | 8 |
| Deletion Start | 119,666,544 |
| Deletion End | 119,686,336 |
| Deletion Size | 19,793 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 119,666,568 | 119,666,588 | CGGGCTCTGAGTTTCCTGTTG |
| TUP | 119,666,589 | 119,666,611 | CTACTGGGCTCCAGTTCTGCCCA |
| TUR | 119,666,649 | 119,666,669 | GGCACTTACTCGGGACTTCTC |
| TDF | 119,686,201 | 119,686,220 | GGCCAGGGTAGGCATTCAAG |
| TDP | 119,686,221 | 119,686,248 | CAGAGCATCTGTTCTAATGGACTGGGCA |
| TDR | 119,686,274 | 119,686,291 | CCAGGCTCTGCCACATTC |
PCR Primers
| Primer | Sequence |
| SU | AGCACAGCCCATCCTAACTG |
| SD | CCCGGTGTACAGTAAGGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10762A-C4 | VGB6 | C57BL/6NTac | |
| 10762A-H2 | VGB6 | C57BL/6NTac | |
| 10762A-G2 | VGB6 | C57BL/6NTac | |
| 10762A-G7 | VGB6 | C57BL/6NTac | |
