| Gene | Prom2 |
| Chromosome | 2 |
| Deletion Start | 127,541,317 |
| Deletion End | 127,528,733 |
| Deletion Size | 12,585 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 127,540,731 | 127,540,753 | GAGTACGGAAAGGTCTGCTTGAG |
| TUP | 127,540,705 | 127,540,728 | CTTCTACCAGCTGCCTCTCCTGCA |
| TUR | 127,540,646 | 127,540,669 | GAGGTTCTTAGAGAAGGCAGTGTC |
| TDF | 127,529,161 | 127,529,179 | CGCTGTCAAGACCTCCAAG |
| TDP | 127,529,134 | 127,529,155 | CCGTCCCATCCGGAAACGCCTC |
| TDR | 127,529,098 | 127,529,117 | CCTGATGCCCACAGTGATTC |
PCR Primers
| Primer | Sequence |
| SU | AGGTACTCCTGGACGGACT |
| SD | GGTAAGCTAGAGAAGTGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 435 bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | 363 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10763A-B1 | VGB6 | C57BL/6NTac | |
| 10763C-A2 | VGB6 | C57BL/6NTac | |
| 10763C-C5 | VGB6 | C57BL/6NTac | |
