| Gene | Bche |
| Chromosome | 3 |
| Deletion Start | 73,702,088 |
| Deletion End | 73,700,579 |
| Deletion Size | 1,510 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 73,701,629 | 73,701,651 | TCAAACTGGGACCTCTTCTCTAC |
| TUP | 73,701,595 | 73,701,622 | ACGATGGGAAGTTTCTAGCTCGTGTTGA |
| TUR | 73,701,513 | 73,701,532 | TCCTGGAGCATCGGGATTTC |
| TDF | 73,700,853 | 73,700,872 | GTGAGCAGTCACCAGAAGTC |
| TDP | 73,700,823 | 73,700,850 | CCGTGACGCTTTGGATGATGTTATTGGA |
| TDR | 73,700,798 | 73,700,817 | GTGCAGGGCAGATGATGTTG |
PCR Primers
| Primer | Sequence |
| SU | TTCAAAGACAACATCACTTAGG |
| SD | CACTTATAACATTCTGATACAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 378 bp | - | + | + |
| TUF | TUR | 139 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | 409 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10764G-F2 | VGB6 | C57BL/6NTac | |
| 10764I-D8 | VGB6 | C57BL/6NTac | |
| 10764I-G8 | VGB6 | C57BL/6NTac | |
