| Gene | Plac1 |
| Chromosome | X |
| Deletion Start | 53,070,828 |
| Deletion End | 53,070,281 |
| Deletion Size | 548 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 53,070,795 | 53,070,815 | TCCTGGGAGGCACTGTCTTAG |
| TUP | 53,070,768 | 53,070,793 | CGCCTTCATGCTCTTCAGCTACTCGG |
| TUR | 53,070,736 | 53,070,756 | TGTCGAGCACAGCACATTCAC |
| TDF | 53,070,424 | 53,070,444 | GCGAAAGCACCTAGCAACAAC |
| TDP | 53,070,393 | 53,070,422 | AGGAAGTATCATTCTTCGGAGTCGCACCCA |
| TDR | 53,070,367 | 53,070,387 | AGGCTCTGGCAAGGTGAACAC |
PCR Primers
| Primer | Sequence |
| SU | ACATAATCCCCTAATTGCACTTC |
| SD | CCCTAAAAGAGCTATCATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 259 bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | 285 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10766C-A11 | VGB6 | C57BL/6NTac | |
| 10766C-B1 | VGB6 | C57BL/6NTac | |
| 10766C-C10 | VGB6 | C57BL/6NTac | |
| 10766C-G3 | VGB6 | C57BL/6NTac | |
| 10766D-E3 | VGB6 | C57BL/6NTac | |
| 10766E-A5 | VGB6 | C57BL/6NTac | |
