| Gene | Rassf7 |
| Chromosome | 7 |
| Deletion Start | 141,216,731 |
| Deletion End | 141,218,311 |
| Deletion Size | 1,599 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 141,217,026 | 141,217,047 | CAGCGTCTTAGGGAGAAGGAAC |
| TUP | 141,217,050 | 141,217,072 | CAGCTGCTGCCACAGGAGTGTCC |
| TUR | 141,217,118 | 141,217,135 | GTCCGCCTCAGGACAAAC |
| TDF | 141,217,754 | 141,217,774 | ACACAGCCAGTCTCAACTCTC |
| TDP | 141,217,775 | 141,217,797 | CACCTGCTGCACAGGCAGTTTGG |
| TDR | 141,217,808 | 141,217,827 | TGCTCCCTGGTCTGATTCTC |
PCR Primers
| Primer | Sequence |
| SU | CCGTTCCTGGAGTGGAGTG |
| SD | AAGGCAGCACACATACAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 398 bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | 389 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10767A-B2 | VGB6 | C57BL/6NTac | |
| 10767A-B5 | VGB6 | C57BL/6NTac | |
| 10767A-C1 | VGB6 | C57BL/6NTac | |
| 10767A-D2 | VGB6 | C57BL/6NTac | |
| 10767A-E4 | VGB6 | C57BL/6NTac | |
