| Gene | Tubb5 |
| Chromosome | 17 |
| Deletion Start | 35,838,050 |
| Deletion End | 35,834,982 |
| Deletion Size | 3,069 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,837,736 | 35,837,757 | GAGGCTGAGGGACTGTGAAGAG |
| TUP | 35,837,691 | 35,837,718 | TTTCTAACGGTTCGAGGACTGGAGGGAT |
| TUR | 35,837,667 | 35,837,687 | GGCTTTGTCTCGGATCGTTTC |
| TDF | 35,835,207 | 35,835,228 | GGCAGTCACCTTCATTGGAAAC |
| TDP | 35,835,182 | 35,835,206 | AGCACAGCCATCCAGGAGCTCTTCA |
| TDR | 35,835,156 | 35,835,177 | AGCCGTAAACTGCTCAGAGATG |
PCR Primers
| Primer | Sequence |
| SU | CTGGTTGGGTAGGCTTTGTC |
| SD | GGGTAACAACCAGAATGTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 431 bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | 337 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10769E-G10 | VGB6 | C57BL/6NTac | |
| 10769D-E5 | VGB6 | C57BL/6NTac | |
| 10769E-C1 | VGB6 | C57BL/6NTac | |
