| Gene | Prrt1 |
| Chromosome | 17 |
| Deletion Start | 34,629,669 |
| Deletion End | 34,632,318 |
| Deletion Size | 2,650 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,630,260 | 34,630,279 | AACGGAGGCCCATGAGATTC |
| TUP | 34,630,281 | 34,630,308 | CAGGTAACTCCACCTGAAGCAAAGTGGA |
| TUR | 34,630,388 | 34,630,409 | GTCAACCACAACCCTCATTCAG |
| TDF | 34,631,496 | 34,631,518 | GCCTGGAAATGCTAACGAGGATG |
| TDP | 34,631,520 | 34,631,542 | AAACAAGACCGGAAGGGCAGGCA |
| TDR | 34,631,562 | 34,631,584 | TGGAAGTTCCCACACTGTCTTAC |
PCR Primers
| Primer | Sequence |
| SU | AGAAGCAGAGAGAAACCGAG |
| SD | CATAGATGAGGCAGGGCTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 363 bp | - | + | + |
| TUF | TUR | 150 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | 369 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10773A-D11 | VGB6 | C57BL/6NTac | |
| 10773A-G8 | VGB6 | C57BL/6NTac | |
