| Gene | Ap1s1 |
| Chromosome | 5 |
| Deletion Start | 137,043,239 |
| Deletion End | 137,035,689 |
| Deletion Size | 7,551 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 137,043,150 | 137,043,173 | GACAAGGAGAGGAAGAAGATGGTC |
| TUP | 137,043,124 | 137,043,147 | AGAGCTCATGCAAGTCGTCCTGGC |
| TUR | 137,043,102 | 137,043,121 | GCTGCACATTTTGGGCTTTC |
| TDF | 137,036,515 | 137,036,532 | GAGGCCGTCTGTCAGTTC |
| TDP | 137,036,489 | 137,036,513 | CCCTGCTGTCTGGCATCCCTACCTT |
| TDR | 137,036,425 | 137,036,445 | GACTCATCTCCCTGCACCAAG |
PCR Primers
| Primer | Sequence |
| SU | TTGCCTGAGGCTGTACTCG |
| SD | ATGGAAGCCACTGAGCACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 368 bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 108 bp | + | + | - |
| NeoFwd | SD | 335 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10776A-A12 | VGB6 | C57BL/6NTac | |
| 10776B-C11 | VGB6 | C57BL/6NTac | |
