| Gene | Gpt |
| Chromosome | 15 |
| Deletion Start | 76,696,843 |
| Deletion End | 76,699,527 |
| Deletion Size | 2,685 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,697,024 | 76,697,042 | TGCCTTGGGCCACTTACTG |
| TUP | 76,697,043 | 76,697,068 | CCCTTCTGCTAAACTTGGGCTGCTGA |
| TUR | 76,697,088 | 76,697,109 | CTACTGCCTTTGTCCCACATTC |
| TDF | 76,698,679 | 76,698,696 | TCCCAGACCAGCCTCAAC |
| TDP | 76,698,697 | 76,698,718 | TCATGCTCTCCTGGGCAGGTGC |
| TDR | 76,698,721 | 76,698,741 | TCCACATAGCCACCACGAAAC |
PCR Primers
| Primer | Sequence |
| SU | TACCAACTCCCTCTGGCAC |
| SD | AGATGCCGTTTCCAGTGTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 339 bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 63 bp | + | + | - |
| NeoFwd | SD | 300 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10778A-A12 | VGB6 | C57BL/6NTac | |
| 10778B-D1 | VGB6 | C57BL/6NTac | |
| 10778B-F7 | VGB6 | C57BL/6NTac | |
| 10778B-G8 | VGB6 | C57BL/6NTac | |
| 10778C-D6 | VGB6 | C57BL/6NTac | |
| 10778C-E6 | VGB6 | C57BL/6NTac | |
