| Gene | Gm13030 |
| Chromosome | 4 |
| Deletion Start | 138,873,925 |
| Deletion End | 138,868,879 |
| Deletion Size | 5,047 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 138,873,391 | 138,873,413 | TCAGGAAAGAAGGAAGACCAGAC |
| TUP | 138,873,359 | 138,873,384 | CCTGCCAACATGTGAGAATCAGGCCA |
| TUR | 138,873,334 | 138,873,353 | CAGGCCCAATCAGGGAAGTG |
| TDF | 138,869,256 | 138,869,274 | CAGAGGGCTTTGGCAGGTG |
| TDP | 138,869,227 | 138,869,249 | AGCGCTACCTGCTGGGAGCACAG |
| TDR | 138,869,179 | 138,869,200 | TCTAAGTCAGGATGGTGGTCTC |
PCR Primers
| Primer | Sequence |
| SU | AGAGCCGTAGGAACCACCA |
| SD | CAGAAGAATGGGATCTGAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 310 bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | 300 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10779A-G4 | VGB6 | C57BL/6NTac | |
| 10779A-C5 | VGB6 | C57BL/6NTac | |
| 10779A-D7 | VGB6 | C57BL/6NTac | |
| 10779A-D10 | VGB6 | C57BL/6NTac | |
| 10779A-D9 | VGB6 | C57BL/6NTac | |
| 10779A-C1 | VGB6 | C57BL/6NTac | |
