| Gene | Ccr6 |
| Chromosome | 17 |
| Deletion Start | 8,255,968 |
| Deletion End | 8,257,096 |
| Deletion Size | 1,129 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 8,256,040 | 8,256,058 | GGGCCATGCTCCCTAGAAG |
| TUP | 8,256,062 | 8,256,090 | TCAGAAACTTCACCAAGGTATTTGTGCCA |
| TUR | 8,256,110 | 8,256,128 | CCCAGGAGGCCAAAGACAC |
| TDF | 8,256,704 | 8,256,723 | TCGTGATCGCTGTGGTTCTC |
| TDP | 8,256,725 | 8,256,749 | TGTTCCTGGCTTGTCAGATCCCTCA |
| TDR | 8,256,775 | 8,256,794 | CCCACTTTGCCCGTGTTGAC |
PCR Primers
| Primer | Sequence |
| SU | CAGAAGTGACCAGGAAAGCC |
| SD | AGCTGGGTATGGGATCTTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10780A-A9 | VGB6 | C57BL/6NTac | |
| 10780A-F10 | VGB6 | C57BL/6NTac | |
| 10780A-F2 | VGB6 | C57BL/6NTac | |
| 10780A-E3 | VGB6 | C57BL/6NTac | |
| 10780A-G8 | VGB6 | C57BL/6NTac | |
