| Gene | Hey2 |
| Chromosome | 10 |
| Deletion Start | 30,842,645 |
| Deletion End | 30,833,691 |
| Deletion Size | 8,955 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,842,626 | 30,842,645 | AAGCGCCCTTGTGAGGAAAC |
| TUP | 30,842,601 | 30,842,624 | ACCTCCGAAAGCGACCTGGACGAG |
| TUR | 30,842,560 | 30,842,580 | GCTCACCCAGGGTAATTGTTC |
| TDF | 30,833,821 | 30,833,839 | GCCCACCCTTGTCGGTATC |
| TDP | 30,833,800 | 30,833,820 | CGCAGCCTCCAGTCCTCAGCA |
| TDR | 30,833,780 | 30,833,799 | TGTTTGTCCCAGTGCTTGTC |
PCR Primers
| Primer | Sequence |
| SU | CCTCTGCCAACGCCTCTC |
| SD | CAGGAGAACTCAGTTATGGCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 60 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10788A-F11 | VGB6 | C57BL/6NTac | |
| 10788A-H7 | VGB6 | C57BL/6NTac | |
