| Gene | Notch4 |
| Chromosome | 17 |
| Deletion Start | 34,587,995 |
| Deletion End | 34,564,449 |
| Deletion Size | 23,547 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,564,823 | 34,564,846 | GTGAGAAGCAAGAGATGGAGACAG |
| TUP | 34,564,852 | 34,564,873 | AGCCCACCGGCTCCCTTTCTGT |
| TUR | 34,564,899 | 34,564,919 | CTTCAGGAAAGGCCAGCAATG |
| TDF | 34,587,137 | 34,587,154 | GCCGCTGCTGTGAACAAC |
| TDP | 34,587,166 | 34,587,188 | CCGCTCTCTCCTCCAGGCTGGAG |
| TDR | 34,587,206 | 34,587,225 | ACGTGGCTCACCCTACTGTC |
PCR Primers
| Primer | Sequence |
| SU | CCTTCCTGGGTCACAGTAGC |
| SD | CTGGAGAACATGGCCTCATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 89 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10800A-B7 | VGB6 | C57BL/6NTac | |
| 10800A-B2 | VGB6 | C57BL/6NTac | |
| 10800A-D2 | VGB6 | C57BL/6NTac | |
| 10800A-B11 | VGB6 | C57BL/6NTac | |
| 10800A-C3 | VGB6 | C57BL/6NTac | |
| 10800A-G2 | VGB6 | C57BL/6NTac | |
