| Gene | Ifng |
| Chromosome | 10 |
| Deletion Start | 118,445,375 |
| Deletion End | 118,441,158 |
| Deletion Size | 4,218 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 118,441,198 | 118,441,217 | TGGCTGTTTCTGGCTGTTAC |
| TUP | 118,441,218 | 118,441,241 | TGCCACGGCACAGTCATTGAAAGC |
| TUR | 118,441,289 | 118,441,310 | ACCACGGTCAACCAACCACAAG |
| TDF | 118,445,172 | 118,445,192 | CAGCGCCAAGCATTCAATGAG |
| TDP | 118,445,194 | 118,445,216 | TCATCCGAGTGGTCCACCAGCTG |
| TDR | 118,445,223 | 118,445,242 | CGCTTCCTGAGGCTGGATTC |
PCR Primers
| Primer | Sequence |
| SU | GTCTCATCGTCAGAGAGCCC |
| SD | CACATTCGAGTGCTGTCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 113 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10801A-D5 | VGB6 | C57BL/6NTac | |
| 10801A-E6 | VGB6 | C57BL/6NTac | |
| 10801A-G3 | VGB6 | C57BL/6NTac | |
| 10801A-H9 | VGB6 | C57BL/6NTac | |
| 10801A-H12 | VGB6 | C57BL/6NTac | |
