| Gene | Wnt16 |
| Chromosome | 6 |
| Deletion Start | 22,288,492 |
| Deletion End | 22,298,245 |
| Deletion Size | 9,754 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 22,288,854 | 22,288,875 | AAGGAGCTGTGCAAGAGGAAAC |
| TUP | 22,288,876 | 22,288,898 | CGTACCTGCTGCCTAGCATCCGC |
| TUR | 22,288,928 | 22,288,947 | CGTGTCGGAACTGGCTTCTG |
| TDF | 22,298,077 | 22,298,096 | GAGGCGCAGATGGCTGTAAC |
| TDP | 22,298,099 | 22,298,119 | CCTCTGCTGTGGCCGAGGCTA |
| TDR | 22,298,162 | 22,298,183 | GACGTAGCAGCACCAGATAAAC |
PCR Primers
| Primer | Sequence |
| SU | CTTTCTCCGCCTTATCTTGC |
| SD | CCAAATCATCCAAAGGCGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 309 bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | 306 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10802A-F11 | VGB6 | C57BL/6NTac | |
| 10802A-C3 | VGB6 | C57BL/6NTac | |
| 10802A-E2 | VGB6 | C57BL/6NTac | |
| 10802A-D7 | VGB6 | C57BL/6NTac | |
| 10802A-B4 | VGB6 | C57BL/6NTac | |
