| Gene | Actl7b |
| Chromosome | 4 |
| Deletion Start | 56,741,353 |
| Deletion End | 56,740,033 |
| Deletion Size | 1,321 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,741,063 | 56,741,083 | GCTGGAGACAACTTCAAGGAG |
| TUP | 56,741,035 | 56,741,061 | CATACGTAGGCCATGAGCTGCTCAACA |
| TUR | 56,740,974 | 56,740,993 | CAATCCCAGTCCACCACAAC |
| TDF | 56,740,342 | 56,740,362 | CTGGCTTCAAGGAGGAGATGG |
| TDP | 56,740,319 | 56,740,341 | CTGCTAACGTGCTGCTGTGCGGT |
| TDR | 56,740,257 | 56,740,274 | GGGCAGAGGAGACTCAAC |
PCR Primers
| Primer | Sequence |
| SU | GTCAGGCATGGACATGTGAG |
| SD | CTTCCTCTCTGGAAACGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 400 bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | 321 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10808A-C2 | VGB6 | C57BL/6NTac | |
