| Gene | Akap3 |
| Chromosome | 6 |
| Deletion Start | 126,860,232 |
| Deletion End | 126,874,122 |
| Deletion Size | 13,891 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 126,860,326 | 126,860,350 | CAACCAACACCAAGACTGGAAAATG |
| TUP | 126,860,353 | 126,860,377 | AAGGTAACCTTCCCCAGCAGCACTA |
| TUR | 126,860,378 | 126,860,401 | GATGACAGTCCAAATGTCCCTAAC |
| TDF | 126,874,021 | 126,874,040 | CGGTGCTGAGGTACGAGAAG |
| TDP | 126,874,047 | 126,874,074 | CAGCTGGATGAAGCAGTGGGAAATGTCA |
| TDR | 126,874,089 | 126,874,108 | TTTGCCATCAGCCAGTCCAG |
PCR Primers
| Primer | Sequence |
| SU | GGAGAGCTGCAGTTGAGTG |
| SD | AGGGAGATTGCGGTCTTACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 263 bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | 317 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10811A-H5 | VGB6 | C57BL/6NTac | |
| 10811B-E12 | VGB6 | C57BL/6NTac | |
| 10811C-F4 | VGB6 | C57BL/6NTac | |
| 10811C-G11 | VGB6 | C57BL/6NTac | |
| 10811D-E1 | VGB6 | C57BL/6NTac | |
| 10811D-H12 | VGB6 | C57BL/6NTac | |
