10813 Alkbh7 Status: ES cell colonies screened / QC no positives
| Gene | Alkbh7 |
| Chromosome | 17 |
| Deletion Start | 56,997,348 |
| Deletion End | 56,999,089 |
| Deletion Size | 1,742 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 56,997,715 | 56,997,736 | GGGACCAAGTTTCTCTGGTGTG |
| TUP | 56,997,749 | 56,997,772 | TGCTGGGCAATTCGATGAGTGACA |
| TUR | 56,997,807 | 56,997,827 | GGACTCAGCTCTGGCATCAAG |
| TDF | 56,998,479 | 56,998,498 | CCTGACCAGAGCCTGCTATC |
| TDP | 56,998,499 | 56,998,526 | CCCAGTACATGTGTTGGACCTGGAACCT |
| TDR | 56,998,557 | 56,998,577 | ATGCCTTCTGCCCACCTTGAC |
PCR Primers
| Primer | Sequence |
| SU | GGGTGTGCTGTTTGACAAGC |
| SD | TGATATTCTCGTGGCCTTAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 377 bp | - | + | + |
| TUF | TUR | 113 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | 264 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
