10816   Apon     Status: ES cell colonies screened / QC positives

GeneApon
Chromosome10
Deletion Start128,254,458
Deletion End128,255,228
Deletion Size771
Genome Build38
MGI IDMGI:88931
NCBI Gene ID28194
BAC ID170D10
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 10

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF128,254,467128,254,487GCATTGCTTCTTGGCTGTATC
TUP128,254,496128,254,515CCCGGTGACCGCCTTTCCGT
TUR128,254,547128,254,568TGATGTGGGTTCAGTTCTTGTG
TDF128,255,000128,255,021GGCCTCTGTAGTCAATGTCACC
TDP128,255,022128,255,047CAGCACTGTACCATGGAGTCTTGGGA
TDR128,255,058128,255,078CATCAGCCTCCTGGCTACTTC

PCR Primers

PrimerSequence
SUGTTAACCTCTGGAAGGTAGC
SDAGGCCCAGAATTCAGCTGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev395 bp-++
TUFTUR102 bp++-
TDFTDR79 bp++-
NeoFwdSD345 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
10816C-A8VGB6C57BL/6NTac
10816C-B5VGB6C57BL/6NTac
10816C-B9VGB6C57BL/6NTac
10816C-D2VGB6C57BL/6NTac
10816C-E11VGB6C57BL/6NTac
10816C-G4VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
Larger Image

VelociGene Genotyping Strategy

Larger Image