| Gene | Aqp6 |
| Chromosome | 15 |
| Deletion Start | 99,601,392 |
| Deletion End | 99,604,343 |
| Deletion Size | 2,952 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 99,601,914 | 99,601,938 | GGGTTCCTCTCCCATTTAAGCATTC |
| TUP | 99,601,942 | 99,601,968 | TAGGCCAGACAGAAATGGCAACATGGT |
| TUR | 99,601,977 | 99,601,995 | TGTCTCGGTGTCCCATCTG |
| TDF | 99,603,700 | 99,603,718 | TCTGGGTAGGACCGCTCAC |
| TDP | 99,603,725 | 99,603,749 | TGTCCTGGCTTCGCTGATCTACAAC |
| TDR | 99,603,769 | 99,603,787 | CGCTGGGCTACAGTCTTGG |
PCR Primers
| Primer | Sequence |
| SU | ACGTGAGCACTCTGAACCTC |
| SD | CTGGAGTAGATTGAGGGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 352 bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | 265 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10818A-H2 | VGB6 | C57BL/6NTac | |
| 10818A-F6 | VGB6 | C57BL/6NTac | |
| 10818A-C6 | VGB6 | C57BL/6NTac | |
| 10818A-E7 | VGB6 | C57BL/6NTac | |
| 10818A-C11 | VGB6 | C57BL/6NTac | |
| 10818A-B3 | VGB6 | C57BL/6NTac | |
