| Gene | Arl2 |
| Chromosome | 19 |
| Deletion Start | 6,141,098 |
| Deletion End | 6,134,406 |
| Deletion Size | 6,693 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 6,140,601 | 6,140,619 | GTAGGCGTGGACTCGGTTG |
| TUP | 6,140,574 | 6,140,598 | CACAGGTCCCGCTGTCACTTAAGGC |
| TUR | 6,140,542 | 6,140,563 | GTGCCAGTTAATGCGGTGAAAG |
| TDF | 6,134,881 | 6,134,901 | ACTGACGGTGGCACGAGTATG |
| TDP | 6,134,858 | 6,134,880 | TAGCTTCAGAGCCACCCACTGGG |
| TDR | 6,134,834 | 6,134,854 | GCCTCAGCTTGTCCTTTCCAG |
PCR Primers
| Primer | Sequence |
| SU | TCAGCCTCTGTCTGGCATC |
| SD | ATGCCTCCTCCTATAGGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 381 bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | 318 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10820A-D2 | VGB6 | C57BL/6NTac | |
| 10820A-G11 | VGB6 | C57BL/6NTac | |
| 10820A-G5 | VGB6 | C57BL/6NTac | |
