| Gene | Arl9 |
| Chromosome | 5 |
| Deletion Start | 77,004,058 |
| Deletion End | 77,009,476 |
| Deletion Size | 5,419 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 77,004,202 | 77,004,222 | GGCCTTGTCCATACCATAGTG |
| TUP | 77,004,224 | 77,004,251 | TGACTCAGAAAGGCACTGCAACACTTCG |
| TUR | 77,004,262 | 77,004,282 | TCATAACCCTGCCCTAACCTC |
| TDF | 77,009,067 | 77,009,084 | AGCAGTGGAGGAGGGAAC |
| TDP | 77,009,085 | 77,009,110 | TGCAAAGACTTAGACGCTCCAGTACC |
| TDR | 77,009,113 | 77,009,132 | GAGTGCTACACAGCCCAAAG |
PCR Primers
| Primer | Sequence |
| SU | GCTTGACTCTTAGAGCTACC |
| SD | CCTGTCTCAAATAGAGGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 408 bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | 414 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10822A-C4 | VGB6 | C57BL/6NTac | |
| 10822A-D11 | VGB6 | C57BL/6NTac | |
| 10822A-F9 | VGB6 | C57BL/6NTac | |
