| Gene | Atp6v0e2 |
| Chromosome | 6 |
| Deletion Start | 48,537,736 |
| Deletion End | 48,540,141 |
| Deletion Size | 2,406 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 48,538,341 | 48,538,361 | CCCTTCTCCTGTTAGCCTCTC |
| TUP | 48,538,365 | 48,538,392 | TCATGAAAGCCTCACACTTTGCCCTTTG |
| TUR | 48,538,412 | 48,538,430 | CTGGCTGTGCAGTGGATTG |
| TDF | 48,539,388 | 48,539,411 | GTTGTGGACCAGTTCAGTAGTATC |
| TDP | 48,539,412 | 48,539,436 | TGCTCCTAACTGGGCATCAGAATCC |
| TDR | 48,539,485 | 48,539,506 | GATGCCCTGACTTCTGTATGAC |
PCR Primers
| Primer | Sequence |
| SU | CAAACCGACCCAGCCAATC |
| SD | CGTTCCTTGGAAAGCTCCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 357 bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 119 bp | + | + | - |
| NeoFwd | SD | 269 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10826A-D10 | VGB6 | C57BL/6NTac | |
| 10826A-F2 | VGB6 | C57BL/6NTac | |
| 10826A-F11 | VGB6 | C57BL/6NTac | |
