| Gene | Cox5b |
| Chromosome | 1 |
| Deletion Start | 36,691,573 |
| Deletion End | 36,693,745 |
| Deletion Size | 2,173 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,692,319 | 36,692,340 | GGCTTGGATGGGATTAGAGGAG |
| TUP | 36,692,341 | 36,692,363 | TTCCTGTCAGCCAGCGTCTGGTC |
| TUR | 36,692,391 | 36,692,409 | TGCCACTGCACTGCAAAAG |
| TDF | 36,693,065 | 36,693,086 | AGCGTGAAGTGGTCTCCTAGTC |
| TDP | 36,693,089 | 36,693,112 | TGGCAGTTTTGTTGCTGTGTCCGA |
| TDR | 36,693,133 | 36,693,154 | CAGAGGGAGGCTTTCAGCATTC |
PCR Primers
| Primer | Sequence |
| SU | CTACTATGTGGCTGGGACAC |
| SD | TGGGCTGGAGCTCTGTAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 406 bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | 242 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10843A-A4 | VGB6 | C57BL/6NTac | |
| 10843A-A12 | VGB6 | C57BL/6NTac | |
| 10843A-B7 | VGB6 | C57BL/6NTac | |
| 10843A-C12 | VGB6 | C57BL/6NTac | |
| 10843A-E8 | VGB6 | C57BL/6NTac | |
| 10843A-A6 | VGB6 | C57BL/6NTac | |
