| Gene | Cno |
| Chromosome | 5 |
| Deletion Start | 36,748,642 |
| Deletion End | 36,747,975 |
| Deletion Size | 668 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,748,431 | 36,748,448 | CTACGCCTCCAGCCTGCT |
| TUP | 36,748,407 | 36,748,424 | CGCGGGACCGAGGCCAGA |
| TUR | 36,748,344 | 36,748,363 | ATGCCCACGAACTCGTCCAC |
| TDF | 36,748,118 | 36,748,137 | GGAGGCTGCTGCATACCATC |
| TDP | 36,748,093 | 36,748,115 | CGTCCCCGCTCTCTTCAGGTCGG |
| TDR | 36,748,029 | 36,748,049 | AAGTGGTCCTCGGTCCGAAAC |
PCR Primers
| Primer | Sequence |
| SU | TGTCACCCTCTTTGGATTAC |
| SD | CAAGGAAACACTGTTCACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 328 bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | 371 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10844B-B7 | VGB6 | C57BL/6NTac | |
| 10844B-E10 | VGB6 | C57BL/6NTac | |
| 10844B-B9 | VGB6 | C57BL/6NTac | |
| 10844B-A4 | VGB6 | C57BL/6NTac | |
