| Gene | Zcchc13 |
| Chromosome | X |
| Deletion Start | 103,630,656 |
| Deletion End | 103,631,185 |
| Deletion Size | 530 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 103,630,804 | 103,630,826 | CGATGTTTGTTATCGCTGTGGTG |
| TUP | 103,630,831 | 103,630,854 | TGGTCACTACGCCAAGGACTGTGA |
| TUR | 103,630,876 | 103,630,894 | TCCTCTTCTCCCGCAGTTG |
| TDF | 103,631,082 | 103,631,100 | GCCACATGGCGGTGAACTG |
| TDP | 103,631,102 | 103,631,125 | AGCAAGACGAGCGAAGTCAGCTGC |
| TDR | 103,631,142 | 103,631,161 | TTCCCGAGCCAGATGTCCAG |
PCR Primers
| Primer | Sequence |
| SU | AGCCCAAAGGCTTTGTGATG |
| SD | TGTCAAAACGAACCGTACTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 289 bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | 335 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10845B-F3 | VGB6 | C57BL/6NTac | |
| 10845B-F5 | VGB6 | C57BL/6NTac | |
| 10845B-F6 | VGB6 | C57BL/6NTac | |
