| Gene | Commd2 |
| Chromosome | 3 |
| Deletion Start | 57,651,650 |
| Deletion End | 57,646,660 |
| Deletion Size | 4,991 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 57,651,358 | 57,651,380 | AGTGCGATCCATTCTCTTTCTCC |
| TUP | 57,651,331 | 57,651,356 | CAGGAGTCTTTGCAGGTCCTAGGGTC |
| TUR | 57,651,284 | 57,651,303 | CGCCTTGACTGACTGCTCTG |
| TDF | 57,647,577 | 57,647,594 | CCACGCCCAGCATGAATG |
| TDP | 57,647,540 | 57,647,566 | TATTTGGCTCTTTGAGACAGGGCCCCA |
| TDR | 57,647,517 | 57,647,535 | CTAGGCCAGCCAAGGCTAC |
PCR Primers
| Primer | Sequence |
| SU | ACACTTTTGCAGGATGGCTC |
| SD | CACAGAAATCAGCAGTATTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 367 bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | 313 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10846A-D12 | VGB6 | C57BL/6NTac | |
| 10846A-D6 | VGB6 | C57BL/6NTac | |
| 10846A-E1 | VGB6 | C57BL/6NTac | |
| 10846B-A11 | VGB6 | C57BL/6NTac | |
| 10846C-H8 | VGB6 | C57BL/6NTac | |
| 10846B-A9 | VGB6 | C57BL/6NTac | |
| 10846B-C6 | VGB6 | C57BL/6NTac | |
| 10846B-E1 | VGB6 | C57BL/6NTac | |
| 10846B-E5 | VGB6 | C57BL/6NTac | |
