| Gene | Stoml3 |
| Chromosome | 3 |
| Deletion Start | 53,507,654 |
| Deletion End | 53,488,796 |
| Deletion Size | 18,859 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 53,489,665 | 53,489,684 | TCCGCAAGTAGGCATGTCAG |
| TUP | 53,489,686 | 53,489,710 | ACTGAGAGGCTGCAAGCATTAGGCA |
| TUR | 53,489,742 | 53,489,760 | GGAGGCAGGAAGCTCAGTG |
| TDF | 53,507,042 | 53,507,061 | GGCTTGCTCAGGTTCCTTTC |
| TDP | 53,507,065 | 53,507,088 | TACAGCTCAGGACCATCCGCCCAG |
| TDR | 53,507,100 | 53,507,119 | AGCTCCCAGCCCTCTGTCAC |
PCR Primers
| Primer | Sequence |
| SU | TTCTTACTGCGACGTGTTGG |
| SD | AGGGTCCTTCTCCAACACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10849A-B11 | VGB6 | C57BL/6NTac | |
| 10849A-F10 | VGB6 | C57BL/6NTac | |
| 10849A-D2 | VGB6 | C57BL/6NTac | |
