| Gene | Dnajb3 |
| Chromosome | 1 |
| Deletion Start | 88,205,675 |
| Deletion End | 88,204,887 |
| Deletion Size | 789 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,205,574 | 88,205,594 | AAGTGGCACCCGGACAAGAAC |
| TUP | 88,205,553 | 88,205,573 | CCCGAGCACAAGGAGGAGGCC |
| TUR | 88,205,533 | 88,205,552 | ACCTGCTTGAACCGCCTCTC |
| TDF | 88,205,188 | 88,205,211 | CGCTTCGTTAGATACTGGATTCAC |
| TDP | 88,205,155 | 88,205,177 | TCTCCAGGAAACTCGGGCCTTTC |
| TDR | 88,205,133 | 88,205,152 | GCCGCAAGACATGGAGAAAG |
PCR Primers
| Primer | Sequence |
| SU | ATACTAGACTCGGTCCTGTC |
| SD | AACAGTGTTTCCTAGCACTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 328 bp | - | + | + |
| TUF | TUR | 62 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 293 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10851A-D11 | VGB6 | C57BL/6NTac | |
| 10851A-E10 | VGB6 | C57BL/6NTac | |
