| Gene | Dok3 |
| Chromosome | 13 |
| Deletion Start | 55,528,511 |
| Deletion End | 55,523,286 |
| Deletion Size | 5,226 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,528,040 | 55,528,060 | ACACCCACAGTTGAGCTACAG |
| TUP | 55,528,017 | 55,528,038 | TGCGCCTGGGCAGATAAACCGT |
| TUR | 55,527,990 | 55,528,014 | GCAAATGACATCTAGCGAAGGAAAC |
| TDF | 55,524,243 | 55,524,262 | TTAGCTGGCCCTACCGTTTC |
| TDP | 55,524,220 | 55,524,241 | TGCGCAAGTACGGCTCTGACAA |
| TDR | 55,524,172 | 55,524,193 | TTACCACCTTTCCAGACAGACC |
PCR Primers
| Primer | Sequence |
| SU | GATCCAGGTGCATGTGCAAG |
| SD | AGTCAGGCTCAGGACACTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 401 bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | 293 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10852A-F5 | VGB6 | C57BL/6NTac | |
| 10852A-H7 | VGB6 | C57BL/6NTac | |
| 10852B-B3 | VGB6 | C57BL/6NTac | |
| 10852B-B5 | VGB6 | C57BL/6NTac | |
| 10852B-C4 | VGB6 | C57BL/6NTac | |
| 10852B-F1 | VGB6 | C57BL/6NTac | |
| 10852B-F11 | VGB6 | C57BL/6NTac | |
| 10852B-G6 | VGB6 | C57BL/6NTac | |
