10853   Eid1     Status: ES cell colonies screened / QC positives

GeneEid1
Chromosome2
Deletion Start125,673,177
Deletion End125,673,671
Deletion Size495
Genome Build38
MGI IDMGI:1889651
NCBI Gene ID58521
BAC ID308C18
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 2

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF125,673,218125,673,238GCTGTACGAAGAAAGCAACGA
TUP125,673,240125,673,260TTGCAGATGGACGTGCTGCCC
TUR125,673,262125,673,280ACCTCCATATAGCCCTCGC
TDF125,673,535125,673,552TGGATGGCGGCTTTCAGG
TDP125,673,555125,673,579CGGTGTGAGAAGAGCCCTTTCGACC
TDR125,673,643125,673,667TCTCGATCAATGATCTCATCACAAC

PCR Primers

PrimerSequence
SUGTCACCAGCGGAAGCATCG
SDTGTGAGACCAAAGAGGAACG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev511 bp-++
TUFTUR63 bp++-
TDFTDR133 bp++-
NeoFwdSD320 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
10853A-A7VGB6C57BL/6NTac
10853A-F7VGB6C57BL/6NTac
10853C-A11VGB6C57BL/6NTac
10853C-B5VGB6C57BL/6NTac
10853C-B12VGB6C57BL/6NTac
10853C-B6VGB6C57BL/6NTac
10853C-D8VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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